Cloning and expression of chicken erythrocyte transglutaminase

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9804-8. doi: 10.1073/pnas.89.20.9804.

Abstract

We report the sequences of cDNAs encoding chicken erythrocyte transglutaminase (EC 2.3.2.13). The complete mRNA consists of 3345/3349 nucleotides and predicts a single open reading frame. Nine peptide sequences derived from partial digests of the isolated protein agreed with the corresponding translation of the open reading frame. Approximately 60% identities between the avian protein and three related mammalian enzymes were found. Chicken erythrocyte transglutaminase mRNA is most abundant in red blood cells and kidney, and it accumulates during erythroid cell differentiation.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens
  • Cloning, Molecular
  • DNA / genetics
  • Erythrocytes / enzymology*
  • Erythropoiesis
  • Gene Expression
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Sequence Alignment
  • Transglutaminases / genetics*

Substances

  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA
  • Transglutaminases

Associated data

  • GENBANK/L02270