Improvement in HLA-DQB typing by PCR-RFLP: introduction of a constant restriction site in one of the primers for digestion control

Tissue Antigens. 1992 Aug;40(2):86-9. doi: 10.1111/j.1399-0039.1992.tb01964.x.


The PCR-RFLP method previously reported by Inoko is a powerful technique for HLA class II typing. The reliability of RFLP interpretation depends on complete digestion by restriction endonucleases using a modified primer with restriction sites as an internal digestion control. The use of restriction enzymes which recognize specific HLA DQB allelic variations makes HLA DQB genotyping possible.

MeSH terms

  • Alleles
  • Base Sequence
  • DNA / genetics
  • Genotype
  • HLA-DQ Antigens / genetics*
  • HLA-DQ beta-Chains
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length


  • HLA-DQ Antigens
  • HLA-DQ beta-Chains
  • HLA-DQbeta antigen
  • DNA