Isolation of [Pro2,Met13]somatostatin-14 and somatostatin-14 from the frog brain reveals the existence of a somatostatin gene family in a tetrapod

H Vaudry; N Chartrel; J M Conlon

doi:10.1016/0006-291x(92)92409-q

Isolation of [Pro2,Met13]somatostatin-14 and somatostatin-14 from the frog brain reveals the existence of a somatostatin gene family in a tetrapod

Biochem Biophys Res Commun. 1992 Oct 15;188(1):477-82. doi: 10.1016/0006-291x(92)92409-q.

Authors

H Vaudry¹, N Chartrel, J M Conlon

Affiliation

¹ European Institute for Peptide Research, CNRS URA 650, UA INSERM, University of Rouen, Mont-Saint-Aignan, France.

PMID: 1358069
DOI: 10.1016/0006-291x(92)92409-q

Abstract

Two somatostatin-related peptides were isolated in pure form from an extract of the brain of the European green frog, Rana ridibunda. The primary structure of the most abundant component was identical to that of mammalian somatostatin-14. The primary structure of the second component, present in approximately 5% of the abundance of somatostatin-14, was established as Ala-Pro-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Met-Cys. This sequence shows two substitutions (Pro for Gly2 and Met for Ser13) compared with mammalian somatostatin-14. The data provide evidence for a somatostatin gene family in tetrapods as well as in teleost fish.

MeSH terms

Amino Acid Sequence
Animals
Brain Chemistry*
Chromatography, High Pressure Liquid
Molecular Sequence Data
Multigene Family*
Rana ridibunda / genetics*
Somatostatin / analogs & derivatives*
Somatostatin / chemistry
Somatostatin / genetics
Somatostatin / isolation & purification*

Substances

somatostatin, Pro(2)-Met(13)-
Somatostatin