Retinal on-bipolar cells contain a nitric oxide-sensitive guanylate cyclase

Neuroreport. 1992 Oct;3(10):845-8. doi: 10.1097/00001756-199210000-00006.


Retinal on-bipolar cells possess specialized glutamate receptors which are coupled via a G-protein to the control of a cyclic GMP (cGMP) cascade. Whole-cell voltage clamp recordings were obtained from light-responsive on-bipolar cells in retinal slices of the dogfish. Inclusion of nitroprusside in the patch-pipette solution induced effects in on-bipolar cells which were consistent with a rise in intracellular cGMP and thus stimulation of guanylate cyclase (GC) activity. Conversely, the soluble GC inhibitors, methylene blue and ferricyanide, induced effects consistent with a fall in intracellular cGMP. Activators of particulate GC had no effect. We conclude that cGMP synthesis in on-bipolar cells is catalysed by a NO-sensitive cyclase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Neoplasm / pharmacology
  • Calcium-Binding Proteins / pharmacology
  • Cyclic GMP / pharmacology
  • Dogfish / physiology*
  • Eye Proteins*
  • Ferricyanides / pharmacology
  • Guanylate Cyclase / metabolism*
  • Hippocalcin
  • In Vitro Techniques
  • Lipoproteins*
  • Methylene Blue / pharmacology
  • Nerve Tissue Proteins*
  • Nitric Oxide / pharmacology*
  • Nitroprusside / pharmacology
  • Recoverin
  • Retina / cytology*
  • Retina / drug effects


  • Antigens, Neoplasm
  • Calcium-Binding Proteins
  • Eye Proteins
  • Ferricyanides
  • Lipoproteins
  • Nerve Tissue Proteins
  • hexacyanoferrate III
  • Recoverin
  • Hippocalcin
  • Nitroprusside
  • Nitric Oxide
  • Guanylate Cyclase
  • Cyclic GMP
  • Methylene Blue