1. The effects of VX (10 microM) were examined on sympathetic ganglion neurons from the bullfrog using intracellular recording techniques. 2. VX significantly increased the amplitude of the residual EPSP from 4.8 +/- 0.86 mV (n = 4) to 13.7 +/- 1.23 mV (n = 4). 3. VX significantly decreased the membrane potential 5.2 +/- 0.75 mV (n = 6). The input resistance and the duration of the spike afterhyperpolarization (AHP) were also reduced 69.8% and 69.6% of control, respectively. 4. VX increased neuronal excitability greater than 200% (n = 5) of control. 5. The VX-induced neuronal excitability may result from a reduction in the duration of the AHP and contribute to the CNS toxicity.