The insulin-like growth factor 1 receptor is required for the proliferation of hemopoietic cells

Oncogene. 1992 Nov;7(11):2243-8.

Abstract

We have investigated the role of the insulin-like growth factor one (IGF1) receptor and its relationship to the proto-oncogene c-myb in the growth of two types of hemopoietic cells: mitogen-stimulated human peripheral blood mononuclear cells and a human promyelocytic cell line (HL-60). Using the antisense strategy and the reverse transcriptase polymerase chain reaction (RT-PCR), we show that expression of the IGF1 receptor is required for the entry into S phase of both stimulated lymphocytes and HL-60 cells. The inhibition of DNA synthesis by antisense oligomers to the IGF1 receptor RNA is accompanied by an inhibition of the expression of the mRNA for a DNA synthesis gene, proliferating cell nuclear antigen (PCNA), the co-factor of DNA polymerase delta. Inhibition of c-myb expression results in a decrease in IGF1 receptor mRNA levels; on the other hand, inhibition of IGF1 receptor expression has no effect on c-myb mRNA levels. A tentative temporal relationship between these three genes (c-myb, IGF1 receptor, PCNA) is proposed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cell Division
  • DNA / biosynthesis
  • Humans
  • Leukemia, Promyelocytic, Acute / pathology*
  • Leukocytes, Mononuclear / physiology*
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Oligonucleotides, Antisense / pharmacology
  • Oncogenes
  • Proliferating Cell Nuclear Antigen
  • RNA, Messenger / analysis
  • Receptor, IGF Type 1 / analysis
  • Receptor, IGF Type 1 / genetics
  • Receptor, IGF Type 1 / physiology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured

Substances

  • Nuclear Proteins
  • Oligonucleotides, Antisense
  • Proliferating Cell Nuclear Antigen
  • RNA, Messenger
  • DNA
  • Receptor, IGF Type 1
  • Tetradecanoylphorbol Acetate