Restriction fragment length polymorphism in four virulence-associated genes of Listeria monocytogenes

Res Microbiol. Mar-Apr 1992;143(3):281-94. doi: 10.1016/0923-2508(92)90020-o.

Abstract

We observed restriction fragment length polymorphism in 4 genes of Listeria monocytogenes associated with virulence. Using the polymerase chain reaction (PCR) and primers derived from published sequences, we amplified the following genes: hlyA coding for listeriolysin O, iap coding for a putative invasion-associated factor, mpl coding for a metalloprotease, and the prfA gene that positively regulates the hylA gene. PCR-amplified DNA were cut with several restriction endonucleases, and the restriction profiles from 29 strains, representing 6 serovars (serovars 1/2a, 1/2b, 1/2c, 3a, 3b and 4b) were compared. Based on these restriction profiles, the strains were categorized into 2 subgroups: one group contained all 10 strains of serovars 1/2a, 1/2c and 3a, the other group contained all 19 strains of serovars 1/2b, 3b and 4b. This division is in complete agreement with multilocus enzyme electrophoretic analysis data which divide the species into the same 2 subgroups. Whether the differences observed in the nucleotide sequences of the 4 virulence-associated genes for the 2 subgroups of L. monocytogenes represent salient variations in pathogenic mechanisms is not known.

MeSH terms

  • DNA, Bacterial / genetics
  • Electrophoresis
  • Gene Amplification / genetics
  • In Vitro Techniques
  • Listeria monocytogenes / classification*
  • Listeria monocytogenes / genetics
  • Listeria monocytogenes / pathogenicity
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • Restriction Mapping
  • Virulence / genetics

Substances

  • DNA, Bacterial