Heat-shock responsive elements in the induction of the multidrug resistance gene (MDR1)

FEBS Lett. 1992 Apr 13;301(1):37-40. doi: 10.1016/0014-5793(92)80205-u.


The MDR1 gene, considered to be involved in multidrug resistance of cancer cells, is expressed in liver, kidney, small intestine and the blood-brain barrier. We investigated MDR1 gene expression in the well-differentiated hepatoma cell line HepG2 after exposure to several stresses and found that sodium arsenite treatment increased MDR1 gene expression 2.6-fold. Deletion analysis of the MDR1 promoter indicated that the transcriptional activation after exposure to arsenite depends on a 60-bp region containing two heat-shock responsive elements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Carcinoma, Hepatocellular / genetics*
  • Chloramphenicol O-Acetyltransferase / genetics
  • DNA Mutational Analysis
  • DNA, Recombinant / genetics
  • Drug Resistance / genetics*
  • Gene Expression Regulation, Neoplastic*
  • Hot Temperature
  • Liver Neoplasms / genetics*
  • Membrane Glycoproteins / genetics*
  • Regulatory Sequences, Nucleic Acid / genetics*
  • Tumor Cells, Cultured


  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • DNA, Recombinant
  • Membrane Glycoproteins
  • Chloramphenicol O-Acetyltransferase