Endotoxin-induced uveitis in mice. 1. Induction of uveitis and role of T lymphocytes

Jpn J Ophthalmol. 1992;36(3):281-90.


Endotoxin-induced uveitis (EIU) with a high frequency of posterior iris synechiae was induced by the systemic injection of 200 micrograms of endotoxin into C3H/HeN mice, an endotoxin-responsive strain. The cell number and the protein concentration within the aqueous humor began to increase 6 hours after the injection, achieving a peak at 24 hours, and decreased gradually thereafter. Inflammatory cells were observed in the anterior chamber, the vitreous body and near the iris-ciliary body histologically. Most of the inflammatory cells were polymorphonuclear cells. On the other hand, C3H/HeJ mice, an endotoxin-unresponsive strain, showed no increase in either cell number or protein concentration in the aqueous humor after endotoxin administration. Pretreatment of C3H/HeN mice with anti-Thy-1.2 antibody significantly decreased both the cell number and the protein concentration in the aqueous humor and the incidence of the posterior synechiae, as compared with the control group. Anti-CD4 antibody also significantly reduced the severity of EIU, while anti-CD8 antibody had no influence on the disease. Anti-IFN-gamma antibody increased the cell number in the aqueous humor. These observations indicate that T lymphocytes, especially CD4+ T lymphocytes, have an extremely important role in the development of EIU in mice.

MeSH terms

  • Animals
  • Anterior Chamber / immunology
  • Anterior Chamber / pathology
  • Bacterial Toxins
  • CD4-Positive T-Lymphocytes / immunology
  • Disease Models, Animal
  • Endotoxins*
  • Enterotoxins
  • Female
  • Interferon-gamma / immunology
  • Isoantibodies / immunology
  • Leukocyte Count
  • Mice
  • Mice, Inbred C3H
  • Neutrophils / immunology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Regulatory / immunology
  • Uveitis / immunology*
  • Vitreous Body / immunology
  • Vitreous Body / pathology


  • Bacterial Toxins
  • Endotoxins
  • Enterotoxins
  • Isoantibodies
  • anti-Thy antibody
  • salmonella toxin
  • Interferon-gamma