Adipose hormone-sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides

Lipids. 1992 Dec;27(12):950-4. doi: 10.1007/BF02535570.


Rat adipose hormone-sensitive lipase-mediated release of fatty acids from triglycerides was studied in three model systems: i) cultured preadipocytes containing polyunsaturated fatty acid-enriched triglyceride; ii) perfused epididymal fat pads; and iii) in vitro incubations of crude preparations of hormone-sensitive lipase with synthetic triglyceride-analogues as substrates. We found that cultured preadipocytes challenged with 10 microM norepinephrine tended to release more omega 6 and omega 3 polyunsaturated fatty acids than saturated fatty acids. Fat pads perfused with 10 microM norepinephrine preferentially released arachidonate and alpha-linolenate but tended to retain oleate and linoleate. Finally, crude preparations of hormone-sensitive lipase released from the triglyceride-analogue substrates alpha-linolenate twice as fast as oleate. We conclude that rat adipose hormone-sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides. We suggest that this may be a mechanism by which these fatty acids are kept from being trapped in fat depots and maintained in the circulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / drug effects
  • Adipose Tissue / enzymology*
  • Animals
  • Arachidonic Acid / metabolism
  • Cells, Cultured
  • Epididymis / metabolism
  • Fatty Acids, Unsaturated / metabolism*
  • In Vitro Techniques
  • Linolenic Acids / metabolism
  • Male
  • Norepinephrine / pharmacology
  • Oleic Acid
  • Oleic Acids / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Stem Cells / metabolism
  • Sterol Esterase / drug effects
  • Sterol Esterase / metabolism*
  • Substrate Specificity
  • Triglycerides / metabolism*
  • alpha-Linolenic Acid


  • Fatty Acids, Unsaturated
  • Linolenic Acids
  • Oleic Acids
  • Triglycerides
  • alpha-Linolenic Acid
  • Arachidonic Acid
  • Oleic Acid
  • Sterol Esterase
  • Norepinephrine