CD4+ T-lymphocytes and interleukin-5 mediate antigen-induced eosinophil infiltration into the mouse trachea

Am Rev Respir Dis. 1992 Aug;146(2):374-7. doi: 10.1164/ajrccm/146.2.374.


In order to determine the role of CD4+ and CD8+ T-cells and of interleukin-5 (IL-5) in causing antigen-induced eosinophil infiltration into the site of airway late-phase reaction, we examined the effect of the in vivo depletion of CD4+ and CD8+ T-cells on the eosinophil infiltration of the trachea induced by antigen inhalation in mice. We also studied the effect of anti-murine IL-5 monoclonal antibody (mAb) on the antigen-induced eosinophil infiltration in the trachea. The eosinophil infiltration into the trachea of ovalbumin (OVA)-sensitized BALB/c mice began to increase 9 h after OVA inhalation and persisted for more than 48 h. The in vivo depletion of CD4+ T-cells by pretreatment with anti-L3T4 mAb significantly decreased the eosinophil infiltration induced by OVA inhalation in the trachea of sensitized mice. However, the in vivo depletion of CD8+ T-cells by pretreatment with anti-Lyt-2 mAb had no significant effect on OVA-induced eosinophil infiltration in the trachea. Pretreatment with anti-murine IL-5 mAb also decreased OVA-induced eosinophil infiltration in the trachea. In contrast, neither disodium cromoglycate nor a selective antagonist for platelet-activating factor CV-6209 decreased OVA-induced airway eosinophilia in the mouse. Our results provide direct evidence that CD4+ but not CD8+ T-cells mediate antigen-induced eosinophil recruitment in the airways and that IL-5 mediates this eosinophil recruitment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Inhalation
  • Animals
  • Antibodies, Monoclonal / administration & dosage
  • Antibodies, Monoclonal / therapeutic use
  • CD4-Positive T-Lymphocytes / immunology*
  • Cromolyn Sodium / pharmacology
  • Cromolyn Sodium / therapeutic use
  • Eosinophils / drug effects*
  • Eosinophils / immunology
  • Evaluation Studies as Topic
  • Hypersensitivity, Delayed / diagnosis
  • Hypersensitivity, Delayed / drug therapy
  • Hypersensitivity, Delayed / immunology*
  • Injections, Intraperitoneal
  • Interleukin-5 / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin / adverse effects*
  • Platelet Activating Factor / antagonists & inhibitors
  • Pyridinium Compounds / administration & dosage
  • Pyridinium Compounds / therapeutic use
  • T-Lymphocytes, Regulatory / immunology
  • Trachea / immunology*


  • Antibodies, Monoclonal
  • Interleukin-5
  • Platelet Activating Factor
  • Pyridinium Compounds
  • CV 6209
  • Ovalbumin
  • Cromolyn Sodium