Determination of Taxotere in human plasma by a semi-automated high-performance liquid chromatographic method

J Chromatogr. 1992 Nov 6;582(1-2):273-8. doi: 10.1016/0378-4347(92)80333-l.


A rapid, selective and reproducible high-performance liquid chromatographic (HPLC) method with ultraviolet detection was developed for the determination of the anti-cancer agent Taxotere in biological fluids. The method involves a solid-phase extraction step (C2 ethyl microcolumns) using a Varian Advanced Automated Sample Processor (AASP) followed by reversed-phase HPLC. The validated quantitation range of the method is 10-2500 ng/ml in plasma with coefficients of variation < or = 11%. The method is also suitable for the determination of Taxotere in urine samples under the same conditions. The method was applied in a phase I tolerance study of Taxotere in cancer patients, allowing the pharmacokinetic profile of Taxotere to be established.

MeSH terms

  • Antineoplastic Agents, Phytogenic / blood*
  • Antineoplastic Agents, Phytogenic / pharmacokinetics
  • Antineoplastic Agents, Phytogenic / urine
  • Chromatography, High Pressure Liquid / methods*
  • Docetaxel
  • Humans
  • Paclitaxel / analogs & derivatives*
  • Paclitaxel / blood
  • Paclitaxel / pharmacokinetics
  • Paclitaxel / urine
  • Taxoids*


  • Antineoplastic Agents, Phytogenic
  • Taxoids
  • Docetaxel
  • Paclitaxel