Clonal derivatives of a human B-lymphoblastoid cell line producing prolactin--a cytogenetic characterization

Cytotechnology. 1990 Jan;3(1):43-50. doi: 10.1007/BF00365265.


The IM-9-P cell line is a variant of the human B-lymphoblastoid cell line IM-9 which ectopically secretes prolactin (hPRL). The heterogeneous line IM-9-P and three sublines of clonal origin, two of them positive and one negative for PRL gene expression, were subjected to cytogenetic analysis and compared with the reference line IM-9 which showed a normal female diploid karyotype. G-banding revealed several rearrangements in the chromosomes. Nine altered chromosomes including one stable marker chromosome were common to all analysed karyotypes of IM-9-P cells and their clones. A second marker chromosome 'mar2' occurred only in the karyotypes of the hPRL producing clones, but not in the non-producing clone. None of the visible alterations involve chromosome 6 which carries the PRL gene in humans.

MeSH terms

  • B-Lymphocytes / metabolism*
  • B-Lymphocytes / ultrastructure
  • Cell Line*
  • Chromosome Aberrations*
  • Chromosome Banding
  • Cytogenetics
  • Female
  • Gene Expression
  • Humans
  • Infant, Newborn
  • Karyotyping
  • Multiple Myeloma
  • Prolactin / genetics
  • Prolactin / metabolism*


  • Prolactin