Immunologically active polysaccharides of Arnica montana cell cultures

Phytochemistry. 1991;30(4):1141-5. doi: 10.1016/s0031-9422(00)95191-4.


From the nutrition medium of Arnica montana cell cultures two homogeneous polysaccharides, an acidic arabino-3,6-galactan-protein with mean Mr of 100,000 and a neutral fucogalactoxyloglucan with mean Mr of 22,500 have been isolated by DEAE-Sepharose CL-6B and Sephacryl S-400 column chromatography. Their structures were elucidated mainly by methylation analysis, partial acidic and enzymatic hydrolysis and 13C NMR spectroscopy. The fucogalactoxyloglucan shows a pronounced enhancement of phagocytosis in vivo. The arabino-3,6-galactan-protein displays a strong anticomplementary effect and stimulates macrophages to excrete the tumour necrosis factor (TNF alpha).

MeSH terms

  • Carbohydrate Sequence
  • Cells, Cultured
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Molecular Sequence Data
  • Molecular Structure
  • Plants / analysis
  • Plants / immunology
  • Polysaccharides / immunology*
  • Polysaccharides / isolation & purification


  • Polysaccharides