In Vitro Expression of Lac-PTS and Tagatose 1,6-bisphosphate Aldolase Genes From Lactococcus Lactis Subsp. Cremoris Plasmid pDI-21

Appl Microbiol Biotechnol. 1990 Sep;33(6):677-9. doi: 10.1007/BF00604936.

Abstract

A 4.4-kb EcoR1-EcoR1 DNA fragment from the Lactococcus lactis subsp. cremoris plasmid pDI-21 encoded the tagatose 1,6-bisphosphate (TBP) aldolase gene and the Lac-PTS genes. In vitro transcription-translation using Escherichia coli S30 extract showed the synthesis of 41,000-, 23,000- and 12,000-dalton proteins which correspond to the TBP-aldolase, Lac-PTS enzyme II, and factor III proteins respectively.

MeSH terms

  • Aldehyde-Lyases / genetics*
  • Gene Expression Regulation, Enzymologic*
  • Genes, Bacterial*
  • Lactococcus lactis / enzymology
  • Lactococcus lactis / genetics*
  • Lactose Factors / genetics
  • Phosphoenolpyruvate Sugar Phosphotransferase System / biosynthesis
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
  • Plasmids*

Substances

  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • phosphoenolpyruvate-lactose dependent phosphotransferase system
  • Aldehyde-Lyases
  • tagatose 1,6-diphosphate aldolase