Evaluation of foreign gene codon optimization in yeast: expression of a mouse IG kappa chain

Biotechnology (N Y). 1991 Dec;9(12):1386-9. doi: 10.1038/nbt1291-1386.


We have optimized the codons in an immunoglobulin kappa chain gene to those preferred in the yeast Saccharomyces cerevisiae. The mutant and wild type kappa chain genes were each fused with a synthetic invertase signal peptide that also contained only yeast-preferred codons, and expressed in the F762 yeast strain. The use of yeast-preferred codons resulted in a more than 5-fold increase in the rate of synthesis and at least a 50-fold increase in the steady state level of protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotechnology
  • Codon / genetics*
  • DNA / genetics
  • Gene Expression
  • Immunoglobulin kappa-Chains / biosynthesis
  • Immunoglobulin kappa-Chains / genetics*
  • Mice
  • Protein Sorting Signals / genetics
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / genetics*


  • Codon
  • Immunoglobulin kappa-Chains
  • Protein Sorting Signals
  • RNA, Fungal
  • RNA, Messenger
  • DNA