The molecular basis of partitioning in aqueous two-phase systems

Trends Biotechnol. 1991 Nov;9(11):381-8. doi: 10.1016/0167-7799(91)90130-a.


Protein purification based on partition in aqueous two-phase systems has attracted interest for many years. This approach has been advocated as a primary-stage unit operation in downstream processing. In reality, application has been strictly limited through inadequate understanding of the complex molecular forces involved in partitioning processes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Methods
  • Molecular Sequence Data
  • Polymers
  • Proteins / chemistry
  • Proteins / isolation & purification*
  • Salts
  • Solubility
  • Water / chemistry


  • Polymers
  • Proteins
  • Salts
  • Water