The Rad52-Rad59 complex interacts with Rad51 and replication protein A

DNA Repair (Amst). 2003 Oct 7;2(10):1127-34. doi: 10.1016/s1568-7864(03)00121-6.

Abstract

The RAD52 gene is essential for homology-dependent repair of double-strand breaks in Saccharomyces cerevisiae. Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-Rad52-Rad59 complexes. The N-terminal region of Rad52, which is required for self-interaction to form a ring structure, is required for interaction with Rad59. Rad59 also shows self-interaction suggesting the formation of heteromeric and homomeric rings of Rad52 and Rad59. In wild-type cells, we propose the Rad51-Rad52-Rad59 complex is involved in conservative recombination events, including gene conversion and reciprocal recombination, whereas the Rad52-Rad59 complex participates in single-strand annealing.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • DNA / genetics
  • DNA Damage
  • DNA Repair / genetics*
  • DNA Replication
  • DNA, Single-Stranded / genetics
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Precipitin Tests
  • Protein Binding
  • Rad51 Recombinase
  • Rad52 DNA Repair and Recombination Protein
  • Recombination, Genetic
  • Replication Protein A
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*

Substances

  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • RAD52 protein, S cerevisiae
  • RAD59 protein, S cerevisiae
  • Rad52 DNA Repair and Recombination Protein
  • Replication Protein A
  • Saccharomyces cerevisiae Proteins
  • DNA
  • RAD51 protein, S cerevisiae
  • Rad51 Recombinase