Serum from 30 young alligators was examined by free boundary, starch block, and cellulose acetate strip electrophoresis. The patterns obtained showed that the alligator serum proteins differ significantly from those of other chordates in that an alpha-globulin is the major component. The average ratio of alpha-globulin to albumin in the serum is 3.4. In this respect it resembles serum of human beings with renal disease in which the alpha-globulin ratio to albumin ratio reaches 3.0.