Conversion of dethiobiotin to biotin in cell-free extracts of Escherichia coli

Biosci Biotechnol Biochem. 1992 Nov;56(11):1780-5. doi: 10.1271/bbb.56.1780.


We constructed the plasmid pTTB151 in which the E. coli bioB gene was expressed under the control of the tac promoter. Conversion of dethiobiotin to biotin was demonstrated in cell-free extracts of E. coli carrying this plasmid. The requirements for this biotin-forming reaction included fructose-1,6-bisphosphate, Fe2+, S-adenosyl-L-methionine, NADPH, and KCl, as well as dethiobiotin as the substrate. The enzymes were partially purified from cell-free extracts by a procedure involving ammonium sulfate fractionation. Our results suggest that an unidentified enzyme(s) besides the bioB gene product is obligatory for the conversion of dethiobiotin to biotin.

MeSH terms

  • Base Sequence
  • Biotechnology
  • Biotin / analogs & derivatives*
  • Biotin / metabolism*
  • Cell-Free System / metabolism
  • DNA, Bacterial / genetics
  • Enzymes / genetics
  • Enzymes / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Fructosediphosphates / metabolism
  • Genes, Bacterial
  • Molecular Sequence Data
  • Plasmids


  • DNA, Bacterial
  • Enzymes
  • Fructosediphosphates
  • Biotin
  • desthiobiotin
  • fructose-1,6-diphosphate