Fatty acid alteration by a delta 9 desaturase in transgenic tobacco tissue

Biotechnology (N Y). 1992 Jun;10(6):675-8. doi: 10.1038/nbt0692-675.

Abstract

Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas chromatographic analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Base Sequence
  • Chromatography, Gas
  • DNA / genetics
  • Fatty Acids / analysis*
  • Fatty Acids, Monounsaturated / analysis*
  • Molecular Sequence Data
  • Nicotiana / chemistry
  • Nicotiana / genetics*
  • Palmitic Acids / analysis
  • Phosphatidylcholines / chemistry
  • Plants, Toxic*
  • Polymerase Chain Reaction
  • Rats
  • Stearoyl-CoA Desaturase / genetics*
  • Stearoyl-CoA Desaturase / metabolism
  • Transfection*

Substances

  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • Palmitic Acids
  • Phosphatidylcholines
  • palmitoleic acid
  • DNA
  • Stearoyl-CoA Desaturase