Fat-body-specific expression of the Drosophila Lsp-2 gene

SAAS Bull Biochem Biotechnol. 1990 Jan;3:129-33.

Abstract

The larval serum protein-2 gene (Lsp-2) of Drosophila melanogaster is expressed at a very high level in the fat body of third-instar larvae. Here we report that Lsp-2 transcription in adult flies produces a unique mRNA localized in the adult adipose tissue of the head in both sexes. To identify regulatory regions of this Drosophila gene, Lsp-2 5'-flanking DNA sequences were fused to the E. coli beta-galactosidase gene (lacZ). Transient expression of the hybrid gene in third-instar larvae indicates that 230 bp just upstream from the 'TATA box' of the Lsp-2 gene are sufficient for larval fat body-specific expression.

MeSH terms

  • Animals
  • Blotting, Northern
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics*
  • Fat Body / metabolism*
  • Female
  • Insect Hormones / biosynthesis
  • Insect Hormones / genetics*
  • Larva
  • Male
  • RNA / metabolism
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Transcription, Genetic
  • Transformation, Genetic

Substances

  • Drosophila Proteins
  • Insect Hormones
  • larval serum protein, Drosophila
  • RNA