The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes

Lett Appl Microbiol. 1991 Sep;13(3):171-4. doi: 10.1111/j.1472-765x.1991.tb00600.x.

Abstract

The linear polymerase chain reaction was used to sequence amplified RNA genes from strains of Bacillus, Thermus and Legionella. The technique described is simple and reproducible and it works well with double standard product which has been PEG precipitated directly from PCR reactions.

MeSH terms

  • Bacillus / genetics*
  • Base Sequence
  • Gene Amplification
  • Legionella / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • RNA, Bacterial / chemistry
  • RNA, Ribosomal, 16S / chemistry*
  • Templates, Genetic
  • Thermus / genetics*

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S