Construction of a umuDC Operon Substitution Mutation in Escherichia Coli

Mutat Res. 1992 Mar;281(3):221-5. doi: 10.1016/0165-7992(92)90012-7.

Abstract

Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The delta (umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.

MeSH terms

  • Bacteriophage lambda / genetics
  • Blotting, Southern
  • Chloramphenicol O-Acetyltransferase / genetics*
  • Chromosome Deletion*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Escherichia coli / genetics*
  • Genes, Bacterial
  • Models, Genetic
  • Operon*
  • Restriction Mapping
  • Transduction, Genetic

Substances

  • DNA, Bacterial
  • Chloramphenicol O-Acetyltransferase