Amplification of viral RNA for the detection of dengue types 1 and 2 virus

J Infect. 1992 Jan;24(1):23-9. doi: 10.1016/0163-4453(92)90842-t.

Abstract

In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types 1 and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Dengue Virus / classification
  • Dengue Virus / genetics
  • Dengue Virus / isolation & purification*
  • Genome, Viral
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • RNA, Viral / genetics*
  • RNA-Directed DNA Polymerase / metabolism
  • Transcription, Genetic / genetics

Substances

  • RNA, Viral
  • RNA-Directed DNA Polymerase