Variation of the O antigen of Campylobacter jejuni in vivo

J Med Microbiol. 1992 Mar;36(3):215-9. doi: 10.1099/00222615-36-3-215.


During the course of a clinical study on patients with campylobacteriosis, three consecutive isolates of Campylobacter jejuni from the same patient were sent for O-serotyping. Marked differences in the specificities of the O antigens of the isolates were observed between the first and third isolates when a passive haemagglutination assay system developed for serotyping C. jejuni was used. Differences in specificity were also demonstrated by immunoblots of lipopolysaccharides (LPS) from proteinase K-digested whole cells. The three isolates could not be distinguished either by restriction endonuclease analysis of chromosomal DNA, by gel electrophoresis of whole-cell proteins or by silver-stained LPS gels, thus providing evidence that they were of the same strain and that antigenic variation had occurred in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigenic Variation
  • Campylobacter Infections / microbiology*
  • Campylobacter jejuni / classification
  • Campylobacter jejuni / genetics
  • Campylobacter jejuni / immunology*
  • Cross Reactions
  • DNA, Bacterial / analysis
  • Diarrhea / microbiology*
  • Electrophoresis, Agar Gel
  • Epitopes / analysis
  • Hemagglutination Tests
  • Humans
  • Immunoblotting
  • Lipopolysaccharides / analysis
  • O Antigens
  • Polysaccharides, Bacterial / immunology*
  • Restriction Mapping
  • Serotyping


  • DNA, Bacterial
  • Epitopes
  • Lipopolysaccharides
  • O Antigens
  • Polysaccharides, Bacterial