Chlamydial rRNA in the joints of patients with Chlamydia-induced arthritis and undifferentiated arthritis

Clin Exp Rheumatol. Jan-Feb 1992;10(1):63-6.

Abstract

Synovial fluid and synovial membrane specimens of 11 patients with Chlamydia-induced arthritis (CIA), 24 patients with undifferentiated arthritis (UndA), 4 patients with post-enteritic reactive arthritis, 3 patients with Lyme arthritis and 9 patients with rheumatoid arthritis were investigated for the presence of Chlamydia trachomatis (C. trachomatis). A single stranded DNA-probe was used for nucleic acid hybridization with ribosomal RNA (rRNA) from C. trachomatis. In 4 patients (CIA = 1, UndA = 3) chlamydial rRNA was found in the synovial fluid. In one additional patient (CIA) the specimen of a synovial membrane biopsy was positive for chlamydial rRNA. The detection of intra-articular chlamydial rRNA is discussed as an indicator for the presence of viable Chlamydiae in inflamed joints.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Infectious / genetics*
  • Arthritis, Infectious / pathology
  • Arthritis, Infectious / physiopathology
  • Biopsy
  • Chlamydia Infections / genetics*
  • Chlamydia Infections / pathology
  • Chlamydia Infections / physiopathology
  • Chlamydia trachomatis / genetics*
  • Chlamydia trachomatis / isolation & purification
  • Chlamydia trachomatis / physiology
  • DNA Probes
  • Humans
  • Joints / chemistry*
  • Joints / microbiology
  • Joints / pathology
  • Nucleic Acid Hybridization
  • RNA, Bacterial / analysis*
  • RNA, Ribosomal / analysis*
  • Synovial Fluid / chemistry
  • Synovial Fluid / microbiology
  • Synovial Membrane / chemistry
  • Synovial Membrane / microbiology
  • Synovial Membrane / pathology

Substances

  • DNA Probes
  • RNA, Bacterial
  • RNA, Ribosomal