Induced RAW 264.7 macrophages express soluble and particulate nitric oxide synthase: inhibition by transforming growth factor-beta

Eur J Pharmacol. 1992 Feb 13;225(2):161-5. doi: 10.1016/0922-4106(92)90096-e.


RAW 264.7 macrophages induced with lipopolysaccharide and interferon-gamma expressed nitric oxide (NO) synthase. Approximately two-thirds of the total induced NO synthase activity was found in the cytosolic fraction, whereas one-third was associated with the particulate fraction. Both enzymes formed L-citrulline in addition to NO-like material. NO and L-citrulline formation by both enzymes were calcium-independent and inhibited by NG-nitro-L-arginine and NG-methyl-L-arginine. Transforming growth factor-beta 1 prevented the induction of both enzymes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / metabolism*
  • Cells, Cultured
  • Citrulline / biosynthesis
  • Interferon-gamma / pharmacology
  • Lipopolysaccharides
  • Macrophages / drug effects
  • Macrophages / enzymology*
  • Nitric Oxide Synthase
  • Transforming Growth Factor beta / pharmacology*


  • Lipopolysaccharides
  • Transforming Growth Factor beta
  • Citrulline
  • Interferon-gamma
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases