Inhibition of myeloid differentiation by the helix-loop-helix protein Id

Science. 1992 Mar 27;255(5052):1700-2. doi: 10.1126/science.1372755.


Id is a helix-loop-helix (HLH) protein that represses activity of several basic helix-loop-helix (bHLH) proteins involved in cell type--specific transcription and cell lineage commitment. The myeloid precursor cell line 32DC13(G) expressed Id messenger RNA, which was transiently decreased when cells were induced to terminally differentiate with granulocyte--colony-stimulating factor. Concomitant with the decrease of Id messenger RNA was the appearance in nuclear extracts of DNA binding proteins that recognized a canonical E-box motif, a DNA binding site for some bHLH proteins. Constitutive expression of an Id complementary DNA in 32DC13(G) cells blocked their ability to differentiate and to induce E-box-binding activity. These results suggest that Id and, hence, bHLH proteins function in the process of myeloid differentiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Differentiation / drug effects*
  • Cells, Cultured
  • DNA-Binding Proteins / physiology*
  • Gene Expression
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Hematopoiesis / drug effects*
  • In Vitro Techniques
  • Inhibitor of Differentiation Protein 1
  • Interleukin-3 / pharmacology
  • Macromolecular Substances
  • RNA, Messenger / genetics
  • Repressor Proteins*
  • Transcription Factors*


  • DNA-Binding Proteins
  • Inhibitor of Differentiation Protein 1
  • Interleukin-3
  • Macromolecular Substances
  • RNA, Messenger
  • Repressor Proteins
  • Transcription Factors
  • Granulocyte Colony-Stimulating Factor