Detection and localization of Epstein-Barr viral genomes in angioimmunoblastic lymphadenopathy and angioimmunoblastic lymphadenopathy-like lymphoma

Blood. 1992 Apr 1;79(7):1789-95.


We studied 23 cases of angioimmunoblastic lymphadenopathy (AILD) and AILD-like lymphoma for evidence of Epstein-Barr virus (EBV) using the polymerase chain reaction (PCR) and in situ hybridization studies. EBV nucleic acid sequences were found by either PCR or in situ hybridization in 96% of the cases. There was a wide range in the number of EBV-positive cells among the different cases as detected by in situ hybridization. The EBV-positive cells most often possessed nuclei of intermediate to large size. Double-labeling immunohistochemistry/in situ hybridization studies demonstrated that most of the EBV-positive cells expressed the B-lineage antigen CD20 (as detected by L26), with a minority of the EBV-positive cells stained for the T-lineage associated antigen, CD43 (as detected by Leu 22). The abnormally high amounts of EBV found in AILD and AILD-like lymphoma may be a reflection of decreased immunocompetence in these patients. The presence of EBV-positive B cells may explain the presence of B-cell clones found by others as well as the paradoxical occurrence of B-cell lymphoma in a primary T-cell lymphoproliferative disorder.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Antigens, CD / analysis
  • Antigens, CD20
  • Antigens, Differentiation, B-Lymphocyte / analysis
  • DNA, Viral / analysis
  • Female
  • Genes, Viral*
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Immunoblastic Lymphadenopathy / immunology
  • Immunoblastic Lymphadenopathy / microbiology*
  • Immunoblastic Lymphadenopathy / pathology
  • Immunohistochemistry
  • Leukosialin
  • Lymph Nodes / pathology
  • Lymphoma / immunology
  • Lymphoma / microbiology*
  • Lymphoma / pathology
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • RNA, Viral / analysis
  • Sialoglycoproteins / analysis


  • Antigens, CD
  • Antigens, CD20
  • Antigens, Differentiation, B-Lymphocyte
  • DNA, Viral
  • Leukosialin
  • RNA, Viral
  • SPN protein, human
  • Sialoglycoproteins