The AA., emphasizing the importance of ascertain the bronchial source of microorganisms recoverable from sputum, recommend for this purpose the use of properly collected sputum specimens and the application of washing treatment for mucopurulent materials. The aim of the present study has been to verify the effectiveness of mechanical apparatus to homogenize sputum without affecting the viability of resident microorganisms and the usefulness of prior microscopical examination to establish the rate of dilute inocula for the culture.