Immunocytochemical identification of proliferative hepatocytes using monoclonal antibody to proliferating cell nuclear antigen (PCNA/cyclin). Comparison with immunocytochemical staining for DNA polymerase-alpha

Am J Clin Pathol. 1992 May;97(5 Suppl 1):S14-20.


The authors investigated whether immunocytochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA/cyclin) could be used to identify proliferative hepatocytes in frozen sections fixed in a mixture of periodate, lysine, and 2% paraformaldehyde. Paraffin sections also were used, which were fixed in 10% formaldehyde. Specimens of liver tissue were obtained from 27 patients with various hepatic diseases. Hepatocytes that were positive for PCNA/cyclin were observed in both types of substrate specimens. In acute hepatitis and chronic active hepatitis, most hepatocytes that were labeled for PCNA/cyclin were located near necrotic foci. However, in cirrhosis, they were detected most often near fibrotic septa; the number of immunoreactive cells varied greatly in different areas of tissue sections in such cases. In hepatocellular carcinoma, many PCNA/cyclin-positive tumor cells were seen throughout the neoplasms. Hepatocytes that were positive for DNA polymerase-alpha showed a similar distribution pattern in serial sections of study cases.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antibodies, Monoclonal / immunology*
  • Antigens, Neoplasm / immunology
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cell Division
  • DNA Polymerase II / metabolism
  • Female
  • Humans
  • Immunohistochemistry*
  • Liver / pathology*
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • Male
  • Middle Aged
  • Nuclear Proteins / immunology*
  • Proliferating Cell Nuclear Antigen
  • Staining and Labeling


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • DNA Polymerase II