Differential human immunodeficiency virus expression in CD4+ cloned lymphocytes: from viral latency to replication

J Virol. 1992 Jun;66(6):3966-70. doi: 10.1128/JVI.66.6.3966-3970.1992.


By using cloning methodology, 13 CD4+, CD8-, CD45RO+, and CD29+ clones, isolated from human immunodeficiency virus (HIV)-negative donors, have been characterized and tested regarding their susceptibility to two strains of HIV type 1 (HIV-1). Infected clones possess integrated provirus. Only six are able to replicate HIV-1, while seven may normally grow without cytopathic effect and without viral replication. These results argue that all CD4+ lymphocyte clones may be infectable but that a heterogeneity exists regarding their abilities to replicate HIV-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • CD4-Positive T-Lymphocytes / microbiology*
  • CD4-Positive T-Lymphocytes / pathology
  • Clone Cells / microbiology*
  • Clone Cells / pathology
  • HIV Core Protein p24 / metabolism
  • HIV Reverse Transcriptase
  • HIV-1 / growth & development*
  • HIV-1 / pathogenicity
  • Humans
  • RNA, Viral / analysis
  • RNA-Directed DNA Polymerase / metabolism
  • Virus Activation
  • Virus Replication*


  • Antigens, CD
  • HIV Core Protein p24
  • RNA, Viral
  • HIV Reverse Transcriptase
  • RNA-Directed DNA Polymerase