Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope

EMBO J. 1992 Jun;11(6):2345-55.

Abstract

We studied protein sorting signals which are responsible for the retention of reticuloplasmins in the lumen of the plant endoplasmic reticulum (ER). A non-specific passenger protein, previously shown to be secreted by default, was used as a carrier for such signals. Tagging with C-terminal tetrapeptide sequences of mammalian (KDEL) and yeast (HDEL) reticuloplasmins led to effective accumulation of the protein chimeras in the lumen of the plant ER. Some single amino acid substitutions within the tetrapeptide tag (-SDEL, -KDDL, -KDEI and -KDEV) can cause a complete loss of its function as a retention signal, demonstrating the high specificity of the retention machinery. However, other modifications confer efficient (-RDEL) or partial (-KEEL) retention. It is also shown that the efficiency of protein retention is not significantly impaired by an increased ligand concentration in plants. The efficiently retained chimeras (-KDEL, -HDEL and -RDEL) were shown to be recognized by a monoclonal antibody directed against the C-terminus of the mammalian reticuloplasmin protein disulfide isomerase (PDI). The recognized epitope is also present in several putative reticuloplasmins in microsomal fractions of plant and mammalian cells, suggesting that the antibodies recognize an important structural determinant of the retention signal. In addition, data are discussed which support the view that upstream sequences beyond the C-terminal tetrapeptide can influence or may be part of the structure of reticuloplasmin retention signals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Biological Evolution
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Chimera
  • Cloning, Molecular
  • Endoplasmic Reticulum / metabolism*
  • Endoplasmic Reticulum / ultrastructure
  • Epitopes
  • Escherichia coli / genetics
  • Golgi Apparatus / metabolism
  • Golgi Apparatus / ultrastructure
  • Isomerases / metabolism
  • Mammals
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Plants / genetics*
  • Plants / metabolism
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Protein Disulfide-Isomerases
  • Protein Sorting Signals / metabolism
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Nucleic Acid
  • Tobacco / genetics*
  • Tobacco / metabolism

Substances

  • Antibodies, Monoclonal
  • Carrier Proteins
  • Epitopes
  • Protein Sorting Signals
  • Isomerases
  • Protein Disulfide-Isomerases