An endothelial ligand for L-selectin is a novel mucin-like molecule

Cell. 1992 Jun 12;69(6):927-38. doi: 10.1016/0092-8674(92)90612-g.


The adhesive interaction between circulating lymphocytes and the high endothelial venules (HEV) of lymph nodes (LN) is mediated by lymphocyte L-selectin, a member of the selectin family of cell adhesion proteins. Previous work has identified a sulfated 50 kd glycoprotein (Sgp50) as an HEV ligand for L-selectin. We now report the purification of this glycoprotein and the utilization of the derived N-terminal amino acid sequence to clone a cDNA. The predicted sequence reveals a novel, mucin-like molecule containing two serine/threonine-rich domains. The mRNA encoding this glycoprotein is preferentially expressed in LN. Antibodies against predicted peptides immunoprecipitate Sgp50 and stain the apical surface of LN HEV. These results thus define a tissue-specific mucin-like endothelial glycoprotein that appears to function as a scaffold that presents carbohydrates to the L-selectin lectin domain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Adhesion Molecules / metabolism*
  • Cell Adhesion*
  • Cloning, Molecular
  • DNA / genetics
  • Genes
  • L-Selectin
  • Ligands
  • Mice
  • Molecular Sequence Data
  • Mucins / genetics*
  • Mucins / metabolism*
  • Oligodeoxyribonucleotides / chemistry
  • Protein Conformation


  • Cell Adhesion Molecules
  • Ligands
  • Mucins
  • Oligodeoxyribonucleotides
  • L-Selectin
  • sulfated glycoprotein p50
  • DNA

Associated data

  • GENBANK/L05499
  • GENBANK/L08101
  • GENBANK/M87278
  • GENBANK/M93428
  • GENBANK/S72766
  • GENBANK/S72767
  • GENBANK/S72768
  • GENBANK/S72769
  • GENBANK/S72771
  • GENBANK/X71664