Regional variability of colonocyte growth and differentiation in the rat

Anat Rec. 1992 Jul;233(3):409-14. doi: 10.1002/ar.1092330308.


The location of stem cells and the direction of colonocyte migration in the normal rat colonic crypt were investigated using a serial bromodeoxyuridine (BrdU) and [3H]thymidine labeling protocol. The results demonstrate that in the distal colon the stem cells are located in the crypt base and that cells migrate up toward the luminal surface. In the proximal colon, however, the stem cells are located in the midcrypt, and the colonocytes migrate in two directions, up toward the luminal surface and down toward the crypt base.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Bromodeoxyuridine
  • Cell Differentiation
  • Cell Division
  • Colon / cytology*
  • Immunohistochemistry
  • Indoles
  • Male
  • Rats / anatomy & histology*
  • Rats, Inbred F344
  • Staining and Labeling
  • Thymidine


  • Indoles
  • high iron diamine-alcian blue
  • Bromodeoxyuridine
  • Thymidine