Abstract
By means of G-banding techniques, chromosome aberrations were studied in short-term cultures of normal renal parenchymal cells from 45 patients with renal cell carcinoma. Clonal chromosomal aberrations were detected in 29 patients; loss of the Y chromosome as well as trisomy X, 5, 7, 9, 10, 12, and 18 was found. Chromosomes 7 and 10 were involved preferentially. Results of fluorescence in situ hybridization with chromosome 7- and 10-specific DNA probes on non-cultured normal kidney cells suggested that the aberrations developed in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Carcinoma, Renal Cell / pathology*
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Cells, Cultured
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Chromosome Aberrations*
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Chromosome Banding
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Chromosomes, Human, Pair 10 / ultrastructure
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Chromosomes, Human, Pair 7 / ultrastructure
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Clone Cells / pathology*
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DNA Probes
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Female
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Fluorescent Dyes
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Humans
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Kidney / pathology*
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Kidney Neoplasms / pathology*
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Male
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Mosaicism
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Nucleic Acid Hybridization
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X Chromosome / ultrastructure
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Y Chromosome / ultrastructure
Substances
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DNA Probes
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Fluorescent Dyes