Induction of micronuclei in BALB/c-3T3 cells by selected chemicals and complex mixtures

Mutat Res. 1992 Jun 1;279(3):217-22.

Abstract

The genotoxicity of benzo[a]pyrene, cyclophosphamide, 2-aminoanthracene, 2-nitrofluorene, nitrosated coal-dust extracts, and cigarette-smoke condensate were tested with the micronucleus assay using an established mammalian cell line. The results showed that all chemicals and complex mixtures studied induced micronuclei in BALB/c-3T3 cells. These results indicate that BALB/c-3T3 cells are capable of activating certain promutagens and procarcinogens. It seems, therefore, that in addition to cell transformation, the micronucleus assay in BALB/c-3T3 cells without an exogenous activation system may be useful for in vitro studies to detect genotoxic chemicals and complex mixtures.

MeSH terms

  • 3T3 Cells
  • Animals
  • Biotransformation
  • Dose-Response Relationship, Drug
  • Mice
  • Mice, Inbred BALB C
  • Micronuclei, Chromosome-Defective / drug effects*
  • Micronucleus Tests
  • Mutagens

Substances

  • Mutagens