The calcium-binding proteins MRP8 and MRP14 form a membrane-associated heterodimer in a subset of monocytes/macrophages present in acute but absent in chronic inflammatory lesions

Eur J Immunol. 1992 Jul;22(7):1891-7. doi: 10.1002/eji.1830220732.


Monocytes/macrophages expressing an epitope recognized by a monoclonal antibody 27E10 are present in acute but are absent in chronic inflammatory disorders. This report shows that the 27E10 antigen is formed by noncovalent association of the two Ca(2+)-binding proteins MRP8 and MRP14 which belong to the S100 protein family. Identification has been confirmed immunochemically, by matrix-assisted UV-laser desorption/ionization spectrometry and by partial amino acid sequencing. Surface expression of the MRP8/MRP14 complex on a subset of monocytes is reported for the first time and shown to be up-regulated in a Ca(2+)-dependent manner. The 27E10 surface-positive monocytes isolated by cell separation techniques release high amounts of tumor necrosis factor-alpha and interleukin-1 beta in contrast to their 27E10 surface-negative counterparts thus emphasizing their role in inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antibodies, Monoclonal / immunology
  • Calcium-Binding Proteins / analysis*
  • Calgranulin A
  • Calgranulin B
  • Chronic Disease
  • Epitopes / analysis
  • Humans
  • Inflammation / metabolism*
  • Macrophages / chemistry*
  • Monocytes / chemistry*
  • Phenotype


  • Antibodies, Monoclonal
  • Calcium-Binding Proteins
  • Calgranulin A
  • Calgranulin B
  • Epitopes