Rapid and simple method for the identification of apolipoprotein E isomorphic phenotypes from whole serum

Electrophoresis. 1992 Apr;13(4):258-61. doi: 10.1002/elps.1150130152.


For the identification of apolipoprotein E isomorphic phenotypes, fresh or thawed serum was analyzed without prior delipidation or other pretreatment. Using 5% polyacrylamide gels with a 40 mm interelectrode distance, the isoforms were separated by isoelectric focusing in immobilized pH gradients ranging from pH 5 to 6.5, and transferred onto polyvinylidene difluoride membranes by contact blotting for 1 h. The apolipoprotein E isoforms were identified following immunostaining. The electrophoresis required less than 2 h and the entire procedure could be completed within 6 h.

MeSH terms

  • Antibodies, Monoclonal / isolation & purification
  • Apolipoproteins E / blood
  • Apolipoproteins E / genetics*
  • Humans
  • Hydrogen-Ion Concentration
  • Immunoblotting
  • Isoelectric Focusing
  • Phenotype
  • Staining and Labeling
  • Time Factors


  • Antibodies, Monoclonal
  • Apolipoproteins E