Neonatal capsaicin-treatment in mice: effects on pancreatic peptidergic nerves and 2-deoxy-D-glucose-induced insulin and glucagon secretion

J Auton Nerv Syst. 1992 Jun 1;39(1):51-9. doi: 10.1016/0165-1838(92)90250-k.


It is not known whether sensory nerves are involved in the insulin, glucagon or glucose responses to autonomic nerve activation induced by 2-deoxy-D-glucose (2-DG). We therefore treated mice neonatally with capsaicin which permanently destroys sensory afferent nerve fibers. Immunohistochemistry of the pancreas at 13-14 weeks of age revealed a substantial reduction of calcitonin gene-related peptide (CGRP)-immunoreactive nerves and a partial reduction of substance P-immunoreactive nerves. In contrast, no effect was observed on galanin-immunoreactive nerves. At age 10-12 weeks, the mice were injected intravenously with 2-DG (500 mg/kg). In controls, 2-DG stimulated insulin and glucagon secretion and induced hyperglycemia (P less than 0.01). Capsaicin treatment partially reduced the glucose and glucagon responses to 2-DG (P less than 0.01). In contrast, the insulin response to 2-DG was not affected by capsaicin. It is concluded that the mouse pancreas contains capsaicin-sensitive sensory CGRP- and substance P-immunoreactive nerve fibers, whereas the galanin-immunoreactive nerve fibers are not sensitive to capsaicin. Furthermore, capsaicin-sensitive sensory nerve fibers are partially involved in 2-DG-induced glucagon secretion and hyperglycemia, whereas sensory nerves are not involved in 2-DG-induced insulin secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn / physiology*
  • Blood Glucose / metabolism
  • Calcitonin Gene-Related Peptide / immunology
  • Calcitonin Gene-Related Peptide / metabolism
  • Capsaicin / pharmacology*
  • Deoxyglucose / pharmacology*
  • Female
  • Galanin
  • Glucagon / metabolism*
  • Immunohistochemistry
  • Insulin / metabolism*
  • Insulin Secretion
  • Mice
  • Neuropeptides / physiology*
  • Pancreas / drug effects
  • Pancreas / innervation
  • Pancreas / metabolism*
  • Peptides / immunology
  • Peptides / metabolism
  • Substance P / immunology
  • Substance P / metabolism


  • Blood Glucose
  • Insulin
  • Neuropeptides
  • Peptides
  • Substance P
  • Galanin
  • Glucagon
  • Deoxyglucose
  • Calcitonin Gene-Related Peptide
  • Capsaicin