32P-postlabelling analysis of DNA from human tissues

Mutat Res. 1992 Jul;282(3):139-45. doi: 10.1016/0165-7992(92)90087-x.


DNA extracted from human lung, bladder, liver, pancreas, cervix and breast tissue samples taken at autopsy (37 sample sets) was analysed by the nuclease P1 enhancement modification of the 32P-postlabelling assay for levels of aromatic carcinogen DNA adducts. Results were combined with those from a previous study for statistical analysis of 56 sample sets (32 male+24 female). A strong trend was seen for increased adduct levels in the lung DNA of smokers and a weak association for the bladder DNA of smokers compared to non-smokers. Aromatic adducts were also detected in other tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogens / analysis*
  • DNA / analysis*
  • DNA Damage*
  • Female
  • Humans
  • Male
  • Organ Specificity
  • Smoking


  • Carcinogens
  • DNA