Bovine rotavirus segment 5 protein expressed in the baculovirus system interacts with zinc and RNA

J Gen Virol. 1992 Aug;73 ( Pt 8):1931-8. doi: 10.1099/0022-1317-73-8-1931.

Abstract

The cDNA sequence of genomic segment 5 of bovine rotavirus (RF strain) has been inserted into baculovirus transfer vectors, downstream of the polyhedrin promotor. Recombinant baculoviruses containing gene 5 were selected and the protein was expressed to high yields in Spodoptera frugiperda cells. The recombinant protein was inoculated into rabbits and mice to produce specific hyperimmune antisera. The polyclonal antiserum reacted with a protein in rotavirus-infected MA104 cells and with a protein translated in vitro. This serum was also used to confirm that the gene 5 protein is not a structural protein. Recently, the gene 5 product has been predicted to be a zinc finger protein and reported to contain a highly conserved arrangement of cysteine residues; here, we demonstrate that the recombinant gene 5 protein binds zinc and is an RNA-binding protein as are several other zinc finger proteins.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Baculoviridae / genetics*
  • Blotting, Western
  • Capsid / genetics
  • Capsid / metabolism*
  • Cell Line
  • Molecular Sequence Data
  • RNA / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Rotavirus / genetics*
  • Viral Core Proteins / genetics
  • Viral Core Proteins / metabolism*
  • Viral Nonstructural Proteins
  • Zinc / metabolism*
  • Zinc Fingers

Substances

  • RNA-Binding Proteins
  • Recombinant Proteins
  • Viral Core Proteins
  • Viral Nonstructural Proteins
  • RNA
  • Zinc