Simultaneous effects of p-chloroamphetamine, d-fenfluramine, and reserpine on free and stored 5-hydroxytryptamine in brain and blood

J Neurochem. 1992 Sep;59(3):1138-44. doi: 10.1111/j.1471-4159.1992.tb08356.x.


The effects of acute treatment with p-chloramphetamine, d-fenfluramine, and reserpine on intracellular (brain tissue and whole blood) and extracellular (CSF and platelet-free plasma) compartments of 5-hydroxytryptamine (5-HT) in the brain and blood of the same rats have been examined. These treatments affected 5-HT in brain tissue and whole blood similarly (r = 0.823). Reserpine significantly reduced both intracellular pools at 2 and 24 h. p-Chloroamphetamine and d-fenfluramine were more effective on brain tissue 5-HT. The concentration of 5-HT in CSF was significantly increased by all treatments. p-Chloroamphetamine induced a dramatic 70-fold increase of CSF 5-HT, paralleling a 42% decrease in brain tissue. d-Fenfluramine significantly increased CSF 5-HT to 212% of controls and reduced whole brain 5-HT (-23%). The effects of p-chloroamphetamine and d-fenfluramine on 5-HIAA in brain, CSF, and plasma were nonsignificant. Individual values of 5-hydroxyindoleacetic acid (5-HIAA) in CSF and brain were highly correlated (r = 0.855), indicating that CSF 5-HIAA reflects well the concentration of 5-HIAA in brain tissue. Yet the intra- and extracellular concentrations of 5-HIAA were unrelated to the 5-HT changes. This indicates that CSF 5-HIAA does not reflect the active (extracellular) compartment of 5-HT in brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Chromatography, High Pressure Liquid
  • Fenfluramine / pharmacology*
  • Hydroxyindoleacetic Acid / cerebrospinal fluid
  • Male
  • Osmolar Concentration
  • Rats
  • Rats, Inbred Strains
  • Reserpine / pharmacology*
  • Serotonin / blood
  • Serotonin / cerebrospinal fluid
  • Serotonin / metabolism*
  • p-Chloroamphetamine / pharmacology*


  • Fenfluramine
  • Serotonin
  • Hydroxyindoleacetic Acid
  • p-Chloroamphetamine
  • Reserpine