Interleukin-8 inhibits the induction of nitric oxide synthase in rat peritoneal neutrophils

Biochem Biophys Res Commun. 1992 Jul 31;186(2):680-5. doi: 10.1016/0006-291x(92)90800-z.

Abstract

The effect of interleukin(IL)-8 and leukotriene B4 (LTB4) on the induction of nitric oxide (NO) synthase in rat peritoneal neutrophils (PMN) ex vivo was studied. IL-8, but not LTB4, caused concentration-dependent inhibition of the induction of a Ca(2+)-independent NO synthase ex vivo. The effect of IL-8 was not attributable to the synthesis of an inhibitor of this enzyme. These findings suggest complex regulatory control of the induction of NO synthase by cytokines.

MeSH terms

  • Amino Acid Oxidoreductases / biosynthesis*
  • Animals
  • Calcium / pharmacology
  • Cells, Cultured
  • Cytosol / metabolism
  • Enzyme Induction / drug effects
  • Interleukin-8 / pharmacology*
  • Kinetics
  • Leukotriene B4 / pharmacology
  • Male
  • Neutrophils / drug effects
  • Neutrophils / enzymology*
  • Nitric Oxide Synthase
  • Rats
  • Rats, Inbred Strains

Substances

  • Interleukin-8
  • Leukotriene B4
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases
  • Calcium