Identification of Broadly Reactive Continuous Antigenic Determinants of Simian Immunodeficiency Virus Glycoproteins

AIDS Res Hum Retroviruses. 1992 Jun;8(6):1153-64. doi: 10.1089/aid.1992.8.1153.

Abstract

The env polyprotein sequences of several simian immunodeficiency virus (SIV) isolates were analyzed using computer algorithms designed to predict immunologically reactive protein segments. Peptides corresponding to predicted epitopes were synthesized and employed in peptide enzyme-linked immunosorbent assay (ELISA) screening of serum panels from experimentally infected macaques, as well as naturally infected, asymptomatic mangabeys and African green monkeys. Several of the peptides are recognized by a high percentage of antisera from each panel of monkeys indicating that they represent group-specific antigenic determinants of SIV. Several type-specific determinants also were identified. These peptides may be a useful tool for studying the kinetics of SIV glycoprotein-specific immune responses produced by infected and vaccine-protected monkeys at the epitope level.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / immunology
  • Cercocebus
  • Chlorocebus aethiops
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology*
  • Macaca mulatta
  • Molecular Sequence Data
  • Simian Acquired Immunodeficiency Syndrome / immunology*
  • Simian Immunodeficiency Virus / immunology*
  • T-Lymphocytes / immunology
  • Viral Envelope Proteins / chemical synthesis
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies, Viral
  • Epitopes
  • Viral Envelope Proteins