Interleukin-1 beta induces the expression of an isoform of nitric oxide synthase in insulin-producing cells, which is similar to that observed in activated macrophages

FEBS Lett. 1992 Aug 24;308(3):249-52. doi: 10.1016/0014-5793(92)81285-t.


The suppressive and cytotoxic effects of interleukin-1 beta (IL-1 beta) on rodent insulin-producing cells observed in vitro are probably mediated through formation of nitric oxide (NO). In this study we demonstrate that IL-1-induced NO formation in isolated rat islets and insulin-producing HIT cells is more sensitive to inhibition by NG-monomethyl-L-arginine than to inhibition by NG-nitro-L-arginine, thus suggesting that IL-1-exposed insulin-producing cells express an isoform of nitric oxide synthase similar to that present in activated macrophages. Furthermore, IL-1 beta markedly increased the mRNA levels of the inducible macrophage form of nitric oxide synthase in HIT cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Oxidoreductases / biosynthesis*
  • Animals
  • Cell Line
  • Enzyme Induction
  • Humans
  • Interleukin-1 / analogs & derivatives
  • Interleukin-1 / pharmacology*
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / enzymology*
  • Isoenzymes / biosynthesis*
  • Macrophage Activation
  • Macrophages / enzymology*
  • Macrophages / immunology
  • Male
  • Nitric Oxide Synthase
  • Nitrites / metabolism
  • RNA, Messenger / biosynthesis
  • Rats


  • Interleukin-1
  • Isoenzymes
  • Nitrites
  • RNA, Messenger
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases