Multiplex PCR amplification of three microsatellites within the CFTR gene

Genomics. 1992 Aug;13(4):1362-4. doi: 10.1016/0888-7543(92)90071-y.


Multiplex PCR amplification has been developed for three highly polymorphic microsatellites (IVS8CA, IVS17BTA, and IVS17BCA) located in intronic regions of the CFTR (cystic fibrosis (CF) transmembrane conductance regulator) gene. The triplex PCR reaction required different concentrations of each pair of primers and labeling of primers in the same reaction. Total informativity is obtained in 90.25% of couples requiring analysis of polymorphisms, and when triplex microsatellite analysis is combined with analysis for the six most common CF mutations in the Spanish population, informativity reaches more than 99%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cystic Fibrosis / genetics*
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA
  • DNA, Satellite / genetics*
  • Heterozygote
  • Humans
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic


  • CFTR protein, human
  • DNA, Satellite
  • Membrane Proteins
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA