Molecular cloning of rabbit CAP-50, a calcyclin-associated annexin protein

Biochem Biophys Res Commun. 1992 Aug 14;186(3):1227-35. doi: 10.1016/s0006-291x(05)81537-2.


CAP-50 is a member of annexin family proteins which binds specifically to calcyclin in a Ca2+ dependent manner (Tokumitsu. H., Mizutani. A., Minami. H., Kobayashi. R., and Hidaka. H. (1992) J. Biol. Chem. 267,8919-8924). The cDNA representing the rabbit form of this protein has been cloned from rabbit lung cDNA library. Sequence analysis of two overlapping clones revealed a 81-nucleotides 5'-nontranslated region, 1512-nucleotides of open reading frame, a 672-nucleotides 3'-nontranslated region, and a poly(A) tail. Authenticity of the clones was confirmed by comparison of portions of the deduced amino acid sequence with eight sequences of proteolytic peptides obtained from rabbit lung protein. CAP-50 cDNA encodes a 503 residue protein with a calculated M(r) of 54,043 and shows that the protein is composed of four imperfect repeats and hydrophobic N-terminal region. C-terminal region including four imperfect repeats shows 58.1% identity with human synexin (annexin VII), 48.0% identity with annexin I, 47.4% identity with annexin II, 60.1% identity with annexin IV, 54.5% identity with annexin V. Hydrophobic N-terminal region composed of 202 amino acid residues is not homologous with other annexin proteins suggesting that CAP-50 is a novel member of annexin family proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Annexins*
  • Base Sequence
  • Blotting, Northern
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / isolation & purification
  • Cloning, Molecular
  • Gene Library
  • Humans
  • Lung / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Oligodeoxyribonucleotides
  • Poly A / genetics
  • Poly A / isolation & purification
  • Polymerase Chain Reaction
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Rabbits
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • Annexins
  • Calcium-Binding Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • calcyclin-associated protein 50
  • Poly A
  • RNA

Associated data

  • GENBANK/D10883
  • GENBANK/D10916
  • GENBANK/D10917
  • GENBANK/D10918
  • GENBANK/D10919
  • GENBANK/D10920
  • GENBANK/S39218
  • GENBANK/S39219
  • GENBANK/S39220
  • GENBANK/S39221
  • GENBANK/S42968