Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies

Toxicon. 1992 Mar;30(3):239-45. doi: 10.1016/0041-0101(92)90866-4.

Abstract

The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Crotalid Venoms / immunology*
  • Crotalid Venoms / toxicity
  • Epitopes / immunology
  • Group II Phospholipases A2
  • Mice
  • Mice, Inbred BALB C
  • Neurotoxins / immunology*
  • Neurotoxins / toxicity
  • Neutralization Tests
  • Phospholipases A / immunology*
  • Phospholipases A / toxicity
  • Phospholipases A2
  • Reptilian Proteins

Substances

  • Antibodies, Monoclonal
  • Crotalid Venoms
  • Epitopes
  • Neurotoxins
  • Reptilian Proteins
  • Phospholipases A
  • Group II Phospholipases A2
  • Phospholipases A2
  • myotoxin I
  • myotoxin II, Bothrops asper